The higher levels of VWF Ag and FVIII:C in both cases as well as healthy volunteers may be suggestive of a true biological variation of VWF and FVIII in the North Indian population. and stored for anti Cardiolipin Antibody (ACA) and anti beta 2 Glycoprotein 1 (a2GP1) antibody at ??20?C. Checks for Personal computer, PS, AT, LA, VWF Ag, FVIII:C, ACA and a2GP1 were performed in batches within 1?month of storage for both individuals and control samples. Deficiencies of natural coagulation inhibitors like Personal computer, PS and AT were tested in the STA Compact Coagulation analyzer (Diagnostica Stago, Asnieres, France) using the packages from the manufacturer: STA Staclot Personal computer, STA Staclot PS and STA Stachrom ATIII. The clot centered kits were utilized for estimation of practical activity of Personal computer and PS while that for AT was chromogenic. The presence of LA was recognized by two methods which included an in house Kaolin clotting test (KCT) and commercial dilute Russel Viper Venom Test (dRVVT) kits in accordance with the recommended recommendations. The assays for antibodies for ACA and Rabbit Polyclonal to ARHGEF11 a2GP1 were carried out using commercial ELISA packages (Orgentec, GMBh). FVL was tested by Polymerase chain reaction- restriction fragment size polymorphism using restriction endonuclease enzyme. VWF Ag levels were estimated using immunoturbidimetric method in the STA Compact Coagulation analyzer (Diagnostica Stago, Asnieres, France) Doxycycline using the latex immune agglutination test kit. Calibration curves were from the instrument using VWF calibrator with traceability against a secondary reference standard of the International Research Standard 97/586. Element VIII:C activity was measured by one stage clot centered assay on the same coagulation analyzer. Personal computer, PS, AT, VWF Ag, FVIII:C, ACA and a2GP1 screening in the laboratory is assessed by participation in the WHO EQAS programmes for Coagulation and Immunology and Immunochemistry from the UK at regular intervals. The cut-off levels to define deficiencies of Personal computer was 63%, PS was 67% in males and 55% in females and AT was 80%. Low ideals for Personal computer, PS and AT were repeat tested after 4C6?weeks in individuals who were not on any anticoagulants, and results were confirmed. The cut-off levels for ACA IgG and IgM were? ?10?GPLU/ml and? ?7?MPLU/ml respectively and for a2GP1 IgG and IgM were? ?5?IU/ml. Positivity for antiphospholipid antibodies were Doxycycline repeated once after 12?weeks of first sampling and positivity reported only if repeat sample was also positive. Statistical Analysis The statistical analysis was carried out using Statistical Package for Sociable Sciences (SPSS Inc., Chicago, IL, version 18.0 for Windows). Normality of data was checked by steps of KolmogorovCSmirnov checks of normality. Mean for steps of dispersion for VWF Ag and element VIII:C were determined. For the purpose of statistical analysis, Personal computer, PS, AT were classified into deficient and normal levels. LA, ACA, a2GP1 were classified as positive and negative. FVL was classified into normal and heterozygous. Blood group was classified into O and non-O organizations. In the absence of available cut off levels for coagulation factors in literature, the 90th percentile of control subjects was used. Using 90th percentile of control, VWF Ag and FVIII:C levels were classified into elevated and not elevated. Analysis of categorical data was carried out using Chi square test. A value? ?0.05 Doxycycline was taken as significant. Predictors for thromboembolism were recognized on univariate analysis and the significant ones were subjected to multivariate logistic regression analysis. Stratification of FVIII:C and VWF Ag levels was carried out and the odds ratio was determined taking an arbitrary baseline value. Results A total of consecutive 152 individuals with VTE were screened for access into the study, of which 100 met the inclusion criteria. Of these data pertaining to Personal computer, PS, AT, FVL and FVIII:C for 25 instances has been reported previously [2]. One hundred age and sex matched healthy volunteers were sampled. The assessment of the demographic data and risk factors between instances and regulates are depicted in Figs.?1 and ?and2.2. The mean age of instances of VTE was 35.2.
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