Of the ‘criteria’ immunoassays for APS, aCL is the most sensitive while a2GPI antibodies are considered highly specific with low level of sensitivity for APS. samples (50 healthy and 50 infectious disease settings [parvo- and syphilis-IgG/IgM positive]). Results The IgG antibody prevalence for aCL and APhL in Propyl pyrazole triol the APS and PST organizations was similar with marginal variations in medical specificities. In contrast to the aCL IgM ELISA, the APhL test showed improved medical specificities (72% aCL vs 94% APhL in the healthy settings; 38% aCL vs 78% APhL in the infectious disease settings) with implications for improved reliability in the analysis of APS. The overall agreement of the APhL with the aCL or a2GPI for the IgG checks was 89% and 85% respectively, and that of the APhL IgM to the aCL or a2GPI IgM checks was 72% and 86% respectively. Summary Routine use of the APhL IgG/IgM ELISA may considerably reduce the high number of false positives associated with the aCL test without loss in level of sensitivity for APS. Keywords: Anticardiolipin, APhL, antiphospholipid antibodies, method comparison Intro The anti-cardiolipin (aCL) and anti-beta 2 glycoprotein I (a2GPI) IgG and/or IgM immunoassays together with the lupus anticoagulant (LA) test are considered ‘criteria’ laboratory markers for the analysis of certain antiphospholipid syndrome (APS), an autoimmune disorder characterized by pregnancy-related morbidity, arterial and/or venous thrombosis [1-2]. Based on the laboratory recommendations for APS, a confirmed positive result of one immunoassay, i.e. aCL or a2GPI IgG or IgM is sufficient for classifying individuals with vascular thrombosis and/or pregnancy related morbidity as having APS [1]. Of the ‘criteria’ immunoassays for APS, aCL is the most sensitive while a2GPI antibodies are considered highly specific with low level of sensitivity for APS. Even though increased sensitivity of the aCL ELISA makes it a favorable test in the initial diagnostic work-up of APS individuals, their lack of specificity with connected high degree of false positive results constitute both a laboratory and clinical challenge. Indeed, several medical studies as well as systematic review of the literature indicate that IgG isotype of either aCL or a2GPI is definitely more strongly associated with APS than that of IgM [3-8]. The inherent difficulty in the standardization of aCL and a2GPI IgM as well as their unreliability in the context of infectious diseases and interfering substances like IgM rheumatoid element poses significant difficulties in the dedication of this antibody isotype in APS [3,9-15]. The aCL IgM antibodies in particular have been shown to happen in infections such as chronic hepatitis C, leprosy, syphilis, Kala-azar, parvovirus B19 among others [10,12-13,16]. The presence of these antibodies in Rabbit Polyclonal to EDG3 different infectious diseases and the acknowledgement that they do not usually correlate with thrombotic events and/or pregnancy-related morbidity in APS makes screening at 2 time points necessary for differentiation of APS-associated from infection-associated aPL antibodies [1]. Based on these observations, there have been suggestions to replace aCL and a2GPI measurements from routine laboratory determinations with more reliable checks for the analysis of APS [17,18]. Indeed alternative checks to aCL IgG/IgM antibodies and additional potential diagnostic markers for APS have been described [19-23]. Of these, the APhL IgG/IgM as determined by ELISA has been reported to have improved specificity with ideal level of sensitivity for the analysis of APS [19]. The main objective with this study was to evaluate the performance characteristics of the APhL IgG/ IgM ELISA relative to the aCL and a2GPI IgG/ IgM antibody checks. Recognizing the inherent challenge of comparing the APhL assays to the sensitive aCL ELISAs, we wanted to investigate its overall performance in 4 Propyl pyrazole triol four unique groups to reduce selection bias. These organizations included: 16 confirmed APS patients, 85 previously tested samples for aCL and a2GPI IgG/IgM, 50 healthy and 50 infectious disease (syphilis or parvovirus B19 IgG/IgM positive) settings. Our data shows Propyl pyrazole triol comparable performance of the APhL and aCL IgG assays with significant difference in the medical specificities for the IgM isotype. Use of the APhL IgG/IgM ELISA may be useful as an alternate assay to aCL IgG/IgM ELISA without loss of diagnostic accuracy for APS. Materials and methods To evaluate the APhL IgG/IgM assays, we used two groups of samples that experienced previously been tested for APS. Sixteen (16) clinically confirmed APS patient sera from the APLA 2010 damp workshop (Courtesy of Dr. Silvia Pierangeli,.
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